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Improved method for the determination of Aspirin and its metabolites in biological fluids by HPLC: application to human and animal studies
Summary
Type:
HPLC
Application #:
103010
Catalog#:
720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection:
UV, 237nm
Conditions:
eluent: 5mM phosphate buffer pH 2.5 - methanol - acetonitrile (68:16:16)
flow rate 1.3ml/min
Authors:
Mays, D.C. et al.
Source:
J. Chromatogr. 311 (1984) 301–309
Details
Legend: Fig. 1. HPLC chromatograms of an extract of human plasma a) prior to dosing, B) 20min after oral administration of 975mg aspirin. Retention times: gentisic acid (GA) 4.5min, salicyluric acid (SU) 5.
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